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multiplex elisa kit  (Boster Bio)


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    Structured Review

    Boster Bio multiplex elisa kit
    Multiplex Elisa Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+multiplex+kit/Multiplex+ELISA+Kit+For+Mouse+Cytokine+Panel+1/pm41985664-197-14-17
    Average 93 stars, based on 6 article reviews
    multiplex elisa kit - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Multiplex Assay:

    Article Title: Interplay of Neuroinflammation and Gut Microbiota Dysbiosis in Alzheimer's Disease Using Diffusion Kurtosis Imaging Biomarker in 3 × Tg-AD Mouse Models.
    Article Snippet: .. Cytokine levels (IL-1β, IL-6, IFN-γ, and TNFα) were quantified using a multiplex ELISA kit (MEK1016; Boster, Pleasanton, CA, USA). ..

    Article Title: In vivo epigenome editing reduces circulating lipids and attenuates atherosclerosis in mice.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: Interplay of Neuroinflammation and Gut Microbiota Dysbiosis in Alzheimer’s Disease Using Diffusion Kurtosis Imaging Biomarker in 3 × Tg-AD Mouse Models
    Article Snippet: .. Cytokine levels (IL-1β, IL-6, IFN-γ, and TNFα) were quantified using a multiplex ELISA kit (MEK1016; Boster, Pleasanton, CA, USA). ..

    Article Title: Subchronic, low-frequency polystyrene microplastic or nanoplastic exposure elicits molecular perturbations but minimal clinical phenotypes in the mouse gut-brain axis.
    Article Snippet: The global public concerns about microand nanoplastics (MNPs) are on the rise, whereas the mechanisms underlying MNP-mediated toxicity have not been fully elucidated.. In particular, toxic responses in the gut-brain axis of mice varied across studies due to the high variability in experimental exposure designs.. Thus, we employed a mouse model (n = 46) with a standardized exposure regimen of 0.3 mg per treatment, administered twice weekly for 12 consecutive weeks, to systematically evaluate MP (5 μm) and NP (0.5 μm) induced toxicity in both the gut and the brain.

    Article Title: Diffusion kurtosis imaging biomarkers associated with amelioration of neuroinflammation, gray matter microstructural abnormalities, and gut dysbiosis by central thalamic deep brain stimulation in autistic -like young rats.
    Article Snippet: .. Serum concentrations of IL-1β, IL-6, IFN-γ, and TNF-α were quantified using dedicated multiplex ELISAs in 96-well plate sandwich immunoassay format precisely according to the manufacturer’s protocol (Boster Biological Technology, Pleasanton, CA, USA). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Interplay of Neuroinflammation and Gut Microbiota Dysbiosis in Alzheimer's Disease Using Diffusion Kurtosis Imaging Biomarker in 3 × Tg-AD Mouse Models.
    Article Snippet: .. Cytokine levels (IL-1β, IL-6, IFN-γ, and TNFα) were quantified using a multiplex ELISA kit (MEK1016; Boster, Pleasanton, CA, USA). ..

    Article Title: Sodium nitrate regulates senescence accompanied by aortic atherosclerosis in ApoE −/− mice through the miR-34a/FGF-21 axis
    Article Snippet: .. The expression of IL-6 and TNF-α was detected by using an ELISA kit (MEK1016, Boster, United States) according to the manufacturer’s protocol. ..

    Article Title: In vivo epigenome editing reduces circulating lipids and attenuates atherosclerosis in mice.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: Interplay of Neuroinflammation and Gut Microbiota Dysbiosis in Alzheimer’s Disease Using Diffusion Kurtosis Imaging Biomarker in 3 × Tg-AD Mouse Models
    Article Snippet: .. Cytokine levels (IL-1β, IL-6, IFN-γ, and TNFα) were quantified using a multiplex ELISA kit (MEK1016; Boster, Pleasanton, CA, USA). ..

    Article Title: Subchronic, low-frequency polystyrene microplastic or nanoplastic exposure elicits molecular perturbations but minimal clinical phenotypes in the mouse gut-brain axis.
    Article Snippet: The global public concerns about microand nanoplastics (MNPs) are on the rise, whereas the mechanisms underlying MNP-mediated toxicity have not been fully elucidated.. In particular, toxic responses in the gut-brain axis of mice varied across studies due to the high variability in experimental exposure designs.. Thus, we employed a mouse model (n = 46) with a standardized exposure regimen of 0.3 mg per treatment, administered twice weekly for 12 consecutive weeks, to systematically evaluate MP (5 μm) and NP (0.5 μm) induced toxicity in both the gut and the brain.

    Expressing:

    Article Title: Sodium nitrate regulates senescence accompanied by aortic atherosclerosis in ApoE −/− mice through the miR-34a/FGF-21 axis
    Article Snippet: .. The expression of IL-6 and TNF-α was detected by using an ELISA kit (MEK1016, Boster, United States) according to the manufacturer’s protocol. ..



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    Transcriptomic profiling and cytokine production in CIRBP-stimulated primary mouse lung fibroblasts. ( A ) Volcano plot of differentially expressed genes in fibroblasts treated with rmCIRBP vs. control. ( B ) Heatmap of representative upregulated and downregulated genes (fold change > 2.5). ( C , D ) Quantitative PCR analysis of Acta2 and Fn1 mRNA expression in fibroblasts treated with or without rmCIRBP. TGF-β–stimulated fibroblasts were included as a positive control. ( E , F ) Western blot analysis of α-SMA and fibronectin protein expression in fibroblasts treated with or without rmCIRBP. TGF-β–stimulated fibroblasts were included as a positive control. ( G – L ) <t>ELISA</t> quantification of cytokines in the culture supernatants: TGF-β, PDGF, IFN-γ, IL-1β, TNF-α, and IL-6. Data are presented as mean ± SEM. *P < 0.05 using one-way ANOVA and Tukey’s multiple comparison test. rmCIRBP recombinant mouse CIRBP.
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    Transcriptomic profiling and cytokine production in CIRBP-stimulated primary mouse lung fibroblasts. ( A ) Volcano plot of differentially expressed genes in fibroblasts treated with rmCIRBP vs. control. ( B ) Heatmap of representative upregulated and downregulated genes (fold change > 2.5). ( C , D ) Quantitative PCR analysis of Acta2 and Fn1 mRNA expression in fibroblasts treated with or without rmCIRBP. TGF-β–stimulated fibroblasts were included as a positive control. ( E , F ) Western blot analysis of α-SMA and fibronectin protein expression in fibroblasts treated with or without rmCIRBP. TGF-β–stimulated fibroblasts were included as a positive control. ( G – L ) <t>ELISA</t> quantification of cytokines in the culture supernatants: TGF-β, PDGF, IFN-γ, IL-1β, TNF-α, and IL-6. Data are presented as mean ± SEM. *P < 0.05 using one-way ANOVA and Tukey’s multiple comparison test. rmCIRBP recombinant mouse CIRBP.
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    Image Search Results


    Transcriptomic profiling and cytokine production in CIRBP-stimulated primary mouse lung fibroblasts. ( A ) Volcano plot of differentially expressed genes in fibroblasts treated with rmCIRBP vs. control. ( B ) Heatmap of representative upregulated and downregulated genes (fold change > 2.5). ( C , D ) Quantitative PCR analysis of Acta2 and Fn1 mRNA expression in fibroblasts treated with or without rmCIRBP. TGF-β–stimulated fibroblasts were included as a positive control. ( E , F ) Western blot analysis of α-SMA and fibronectin protein expression in fibroblasts treated with or without rmCIRBP. TGF-β–stimulated fibroblasts were included as a positive control. ( G – L ) ELISA quantification of cytokines in the culture supernatants: TGF-β, PDGF, IFN-γ, IL-1β, TNF-α, and IL-6. Data are presented as mean ± SEM. *P < 0.05 using one-way ANOVA and Tukey’s multiple comparison test. rmCIRBP recombinant mouse CIRBP.

    Journal: Scientific Reports

    Article Title: Cold inducible RNA binding protein promotes fibroblast activation and its inhibition represents a potential therapeutic target in pulmonary fibrosis

    doi: 10.1038/s41598-026-39649-3

    Figure Lengend Snippet: Transcriptomic profiling and cytokine production in CIRBP-stimulated primary mouse lung fibroblasts. ( A ) Volcano plot of differentially expressed genes in fibroblasts treated with rmCIRBP vs. control. ( B ) Heatmap of representative upregulated and downregulated genes (fold change > 2.5). ( C , D ) Quantitative PCR analysis of Acta2 and Fn1 mRNA expression in fibroblasts treated with or without rmCIRBP. TGF-β–stimulated fibroblasts were included as a positive control. ( E , F ) Western blot analysis of α-SMA and fibronectin protein expression in fibroblasts treated with or without rmCIRBP. TGF-β–stimulated fibroblasts were included as a positive control. ( G – L ) ELISA quantification of cytokines in the culture supernatants: TGF-β, PDGF, IFN-γ, IL-1β, TNF-α, and IL-6. Data are presented as mean ± SEM. *P < 0.05 using one-way ANOVA and Tukey’s multiple comparison test. rmCIRBP recombinant mouse CIRBP.

    Article Snippet: After 24 h of treatment, cytokine concentrations in the culture supernatants were quantified using the arigoPLEX® Mouse Proinflammatory Cytokine Multiplex ELISA Kit and the arigoPLEX® Mouse Fibrotic Marker Multiplex ELISA Kit (arigo Biolaboratories Corp., Taiwan) according to the manufacturer’s instructions.

    Techniques: Control, Real-time Polymerase Chain Reaction, Expressing, Positive Control, Western Blot, Enzyme-linked Immunosorbent Assay, Comparison, Recombinant

    CIRBP promotes collagen production and proliferation via TLR2/4-dependent IL-6 signaling. ( A ) IL-6 concentrations in the culture supernatants of primary lung fibroblasts treated with rmCIRBP, with or without C23, TLR4-I, or TLR2-I, measured by ELISA. ( B ) Soluble collagen levels in the supernatants following treatment with rmCIRBP ± anti-IL-6 antibody. ( C ) Proliferative activity of fibroblasts assessed by the WST assay after treatment with rmCIRBP and/or anti-IL-6 antibody. ( D ) Soluble collagen production in fibroblasts treated with rmCIRBP and either TLR4-I or TLR2-I. ( E ) Fibroblast proliferation measured using the WST assay following cotreatment with rmCIRBP and TLR4-I or TLR2-I. ( F ) Schematic representation of the proposed mechanism: CIRBP, which is highly expressed in fibrotic lungs, stimulates IL-6 production via TLR2/4, promoting collagen secretion and fibroblast proliferation in an autocrine manner and contributing to the progression of tissue fibrosis. Data are presented as mean ± SEM. *P < 0.05 using one-way ANOVA and Tukey’s multiple comparison test.

    Journal: Scientific Reports

    Article Title: Cold inducible RNA binding protein promotes fibroblast activation and its inhibition represents a potential therapeutic target in pulmonary fibrosis

    doi: 10.1038/s41598-026-39649-3

    Figure Lengend Snippet: CIRBP promotes collagen production and proliferation via TLR2/4-dependent IL-6 signaling. ( A ) IL-6 concentrations in the culture supernatants of primary lung fibroblasts treated with rmCIRBP, with or without C23, TLR4-I, or TLR2-I, measured by ELISA. ( B ) Soluble collagen levels in the supernatants following treatment with rmCIRBP ± anti-IL-6 antibody. ( C ) Proliferative activity of fibroblasts assessed by the WST assay after treatment with rmCIRBP and/or anti-IL-6 antibody. ( D ) Soluble collagen production in fibroblasts treated with rmCIRBP and either TLR4-I or TLR2-I. ( E ) Fibroblast proliferation measured using the WST assay following cotreatment with rmCIRBP and TLR4-I or TLR2-I. ( F ) Schematic representation of the proposed mechanism: CIRBP, which is highly expressed in fibrotic lungs, stimulates IL-6 production via TLR2/4, promoting collagen secretion and fibroblast proliferation in an autocrine manner and contributing to the progression of tissue fibrosis. Data are presented as mean ± SEM. *P < 0.05 using one-way ANOVA and Tukey’s multiple comparison test.

    Article Snippet: After 24 h of treatment, cytokine concentrations in the culture supernatants were quantified using the arigoPLEX® Mouse Proinflammatory Cytokine Multiplex ELISA Kit and the arigoPLEX® Mouse Fibrotic Marker Multiplex ELISA Kit (arigo Biolaboratories Corp., Taiwan) according to the manufacturer’s instructions.

    Techniques: Enzyme-linked Immunosorbent Assay, Activity Assay, WST Assay, Comparison